Monoclonal antibody characterization of Plasmodium falciparum antigens
Campbell, G.H.; Miller, L.H.; Hudson, D.; Franco, E.L.; Andrysiak, P.M.
American Journal of Tropical Medicine and Hygiene 33(6): 1051-1054
1984
ISSN/ISBN: 0002-9637 PMID: 6391218 Document Number: 233424
A set of 8 monoclonal antibodies produced against Plasmodium falciparum was characterized. In an indirect fluorescent antibody assay the antibodies produced small dots of fluorescence in schizonts and individual merozoites. This merozoite-associated dot reactivity occurred with 21 different strains of P. falciparum, but there was no reactivity with other human, nonhuman primate, or rodent Plasmodium species. 3 of the monoclonal antibodies precipitated proteins of molecular weight (mw) 145 000, 135 000 and 104 000. Five of the monoclonal antibodies precipitated proteins of mw 78 000, 63 000, 42 000 and 40 000. We characterized a set of eight monoclonal antibodies produced against Plasmodium falciparum. In an indirect fluorescent antibody assay the antibodies produced small dots of fluorescence in schizonts and individual merozoites. This merozoite-associated dot reactivity occurred with 21 different strains of P. falciparum, but there was no reactivity with other human, nonhuman primate, or rodent Plasmodium species. Three of the monoclonal antibodies precipitated proteins of Mr 145 000, 135 000, and 104 000. Five of the monoclonal antibodies precipitated proteins of Mr 78 000, 63 000, 42 000, and 40 000.AS.