Characterization of Plasmodium falciparum strains of the State of Rondonia, Brazil, using microtests of sensitivity to antimalarials, enzyme typing and monoclonal antibodies
Di Santi, S.M.; Boulos, M.; Vasconcelos, M.A.; Oliveira, S.; Couto, A.; Rosário, V.E.
Revista do Instituto de Medicina Tropical de Sao Paulo 29(3): 142-147
1987
ISSN/ISBN: 0036-4665 PMID: 3324280 Document Number: 299896
Nine samples of Plasmodium falciparum were collected from migrant workers who were infected in the State of Rondonia, Brazil. These samples were maintained in culture for characterization by enzyme typing using acetate cellulose electrophoresis, drug susceptibility to chloroquine, amodiaquine, mefloquine and quinine and antigenic diversity analysis using strain specific monoclonal antibodies. The results obtained have confirmed major variation among all samples studied: it was found consistent resistance to chloroquine moderated resistance to amodiaquine and quinine and susceptibility to low levels of mefloquine; only two isolates have shown susceptibility to all drugs in the in vitro assays. Enzyme typing showed presence of both GPI 1 and 2 and ADA 1 and 2 parasites, while PEP and LDH all samples were type 1. Serotyping with PSA monoclonal antibodies showed presence of three different serotypes (II, III and IV). These results confirm: a) variation among samples for markers analysed; b) in this area and for the small number of samples studied, no significant differences or new types of parasites were seen.