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Characterization of total RNA, messenger poly (A) + RNA and homologous complementary DNA in experimental cardiac hypertrophy

Moalic, J.M.; Swynghedauw, B.; Bouveret, P.; Bercovici, J.; de la Bastie, D.; Schwartz, K.

Archives des Maladies du Coeur et des Vaisseaux 77(11): 1155-1157

1984

ISSN/ISBN: 0003-9683
PMID: 6084494
Document Number: 228677
Two biological modifications are observed during adaptation of cardiac tissue to work overload: an increase in total protein synthesis and a redistribution of myosin isoenzymes. These modifications suggest that changes in DNA transcription are involved in the early response of cardiac tissue to overload. Results are reported in this paper that show a parallel increase in total and polyadenylated RNA content and concentration in cardiac overloaded tissue in the rat. The characterization of cardiac poly (A)+ mRNA by mRNA X cDNA hybridization and the identification of specific mRNAs with recombinant plasmid cDNAs are given in this report as preliminary results.

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