Heterogeneity of the poly (C) tract of encephalomyocarditis virus RNA
Svitkin, I.V.; Krupp, G.; Gross, H.J.
Bioorganicheskaia Khimiia 9(12): 1638-1643
1983
ISSN/ISBN: 0132-3423 PMID: 6091690 Document Number: 198995
The nucleotide sequence of the RNase T1-resistant fragment of encephalomyocarditis virus RNA that includes the poly(C) tract was determined by gel sequencing and mobility shift methods. This sequence is (5') AC126(127) UCUCUCUC9UAACG (3'). The results show that the poly(C) tract is discontinuous, i.e., it is interrupted by the UCUCUCU-sequence. The tract displays anomalously high mobility in polyacrylamide gels as compared to random polynucleotides, indicating that electrophoretic determination of its length gives underestimated values.