Lipid dependence of cytochrome P-450 activity in rat liver microsomes using lipid exchange proteins
Diatlovitskaia, E.V.; Petkova, D.K.; Lemenovskaia, A.F.; Bergel'son, L.D.
Biokhimiia 47(2): 216-220
1982
ISSN/ISBN: 0320-9725 PMID: 7066425 Document Number: 191935
Modification of membrane lipid composition by lipid exchange proteins was used to study the lipid dependence of cytochrome P-450 activity in rat liver microsomes. The introduction of exogenous lysophosphatidylcholine into microsomal membranes decreased the capacity of cytochrome P-450 to demethylate dimethylaniline and dimethylaminoantipyrine and to hydroxylate aniline. A subsequent introduction of phosphatidylcholine resulted in an almost complete reactivation of the enzyme.