Aminoacyl fucosides as possible biochemical markers at tumorigenic and metastatic potential in herpes simplex virus type 2-transformed rat cells
Respess, R.A.; Edwards, I.; Kucera, L.S.; Waite, M.
Cancer Research 41(9 Pt 1): 3507-3511
1981
ISSN/ISBN: 0008-5472 PMID: 6266657 Document Number: 175322
Two classes of aminoacyl fucosides, termed FL3 and FL4, were studied as possible markers of tumorigenic and metastatic potential in herpes simplex virus type 2 transformed rat cells. Clonal cell lines of transformed highly tumorigenic and metastatic (t-REF-G-1.1), nontumorigenic (t-REF-G-2.0) and secondary nontransformed rat embryo fibroblast cells were labeled with [3H]fucose, and cell extracts were analyzed for ratio of radioactivity incorporated into FL3 and FL4. In extracts from t-REF-G-2.0 and nontransformed rat embryo fibroblast cells, the ratios of FL4/FL3 were 5.78 and 5.71, respectively. t-REF-G-2.1 cells exhibited a FL4/FL3 ratio of 1.45 while t-REF-G-1.1 cells exhibited a FL4/FL3 ratio of 0.74. In subclonal cell lines isolated from 12-O-tetradecanoylphorbol-13-acetate-treated and mock-treated t-REF-G-2.1 cells, the FL4/FL3 ratios correlated with the tumorigenic and metastatic potential of these subclones in newborn syngeneic White Buffalo rats. Alterations in fucose-labeled components can be used to predict the tumorigenic and metastatic potential or herpes simplex virus type 2-transformed rat cells.