Potency test of killed polio vaccines. Statistical analysis of test on chicken and preliminary results in combination with ELISA D-antigen determinations

Larbaigt, G.; Moulin, J.; Souvras, M.; Gibelin, N.; Beranger, G.

Developments in Biological Standardization 47: 113-118

1981


ISSN/ISBN: 0301-5149
PMID: 6262141
Document Number: 173235
The authors resume a several years' experience of potency testing inactivated polio vaccines with an in vivo-vitro assay based on inoculation of 3 week-old chicken and measure of neutralizing antibodies at 5 days on Hep 2 cells in a microplate system. Critical points, specificity and reproducibility are successively outlined. Preliminary combining results with determination of D-antigen by ELISA method and human immunization are submitted in view of a future standardization.

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