Potency testing of pertussis immunoglobulins, relationship between neutralisation test and agglutination test

Schneider, W.; Sabin, S.; Schwanig, M.

Developments in Biological Standardization 44: 125-128

1979


ISSN/ISBN: 0301-5149
PMID: 575633
Document Number: 146549
Neutralisation tests were done with pertussis immunoglobulins and immunosera in which mice were immunised by intraperitoneal injection and challenged 4 hours later by intracerebral injection. The results of the neutralisation tests were compared with those of the agglutination tests done with the same preparations. For 2 pertussis immunoglobulins a correlation was found between the results of both tests, but generally no correlation exists between the agglutinating activity and the protective value of the preparations. There is a more than 100-fold difference in their relationship. Therefore a neutralisation test should be used for potency testing of pertussis immunoglobulins.

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