Comparison of the haemocytometer and tube modifications of the leucocyte adherence inhibition assay
Holán, V.; Schimke, R.; Ambrosius, H.; Hasek, M.
Folia Biologica 27(1): 66-75
1981
ISSN/ISBN: 0015-5500 PMID: 6162681 Document Number: 168948
The hemocytometer [HCM] and the tube modifications of the leukocyte adherence inhibition [LAI] assay were compared in guinea pigs immunized with bovine .gamma.-globulin or bovine serum albumin. In the direct test, both modifications yielded positive results. In the indirect test, which is dependent on a cell-free mediator of the lymphokine nature, the HCM LAI assay only gave positive results. The factor produced by immune guinea pig lymphocytes and responsible for LAI reactions in the HCM modification was also able to inhibit adherence of human and rat leukocytes, but not of mouse leukocytes. Evidently the tube and HCM LAI assays have a different mechanism.