Leucocyte adherence inhibition test for the detection of cell-mediated immunity to malignant melanoma
Winter, M.; Nelson, D.S.; Milton, G.W.
Australian and New Zealand Journal of Medicine 10(4): 405-409
1980
ISSN/ISBN: 0004-8291 PMID: 7000060 Document Number: 156081
A tube leukocyte adherence test was used to detect reactivity of melanoma patients to extracts of melanoma tissue. Breast cancer extracts were used as a control. Of 31 melanoma patients, 22 gave a positive reaction (nonadherence index > 25%) to melanoma extracts, whereas only 3 of 24 normal subjects did so. Of 16 patients with stage 1 melanoma, 15 reacted but only 7 of 15 patients with stage 2 or stage 3 disease did so. None of 7 patients with breast cancer reacted to melanoma extract, but 4 of them reacted to breast cancer extract. None of 4 patients with benign breast disease reacted to either extract. Leukocyte adherence inhibition is apparently potentially useful in investigations of tumor-specific immune reactivity to melanoma and of melanoma antigens.