Comparison of the hemocytometer and tube modifications of the leukocyte adherence inhibition assay -- II. Application of both modifications for detection of anti-tumor immunity in man
Schimke, R.; Holán, V.; Sibl, O.; Hasek, M.; Ambrosius, H.
Neoplasma 28(3): 257-264
1981
ISSN/ISBN: 0028-2685 PMID: 7027056 Document Number: 169037
The hemocytometer and tube modifications of the leukocyte adherence inhibition (LAI) assay were compared under identical experimental conditions in patients with carcinoma of the larynx. In the direct test both modifications showed adherence inhibition if patients' peripheral blood leukocytes (PBL) were incubated with a larynx cancer antigen preparation. Cell-free supernatants obtained by cocultivation of patients' PBL with the antigenic extract yielded positive results only in the hemocytometer modification. Those supernatants inhibited the adherence of normal human PBL and guinea pig peritoneal cells. Human encephalitogenic protein used as antigenic preparation in direct and indirect hemocytometer modifications also caused changes in adherence. The hemocytometer and tube LAI assays apparently are based on different mechanisms.