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Analysis of human lymphocyte cell cycle kinetics in vitro by incorporation into DNA of bromodeoxyuridine

Botto Micca, F.; Fasano, M.E.; Ardito, G.; Lamberti, L.

Bollettino della Societa Italiana di Biologia Sperimentale 56(7): 725-731

1980

ISSN/ISBN: 0037-8771
PMID: 7448065
Document Number: 166319
Human lymphocytes of four donors were cultured for 40 to 76 hours and harvested at 4 hour intervals. They were analysed for the number of 1st, 2nd and 3rd division metaphases using the BrdU-Giemsa technique. All 44 hours cultures showed metaphases only in their 1st division; 2nd division metaphases began to appear in 48 hours cultures and 3rd division metaphases in 64 hours cultures. Another group of lymphocytes from 4 other donors were irradiated and harvested at 72 hours. These cultures showed a decrease in the percentage of 3rd division metaphases, accompanied by an increase in the percentage of 1st division metaphases, proportional to the irradiation dose.

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