In vitro decondensation of the human spermatozoan nucleus and image analysis. Quantitative data on the kinetics of in vitro decondensation of the human spermatozoan nucleus
Pasteur, X.; Laget, B.; Azema, J.; Jourlin, M.; Laurent, J.L.
Acta Europaea Fertilitatis 15(3): 185-193
1984
ISSN/ISBN: 0587-2421 PMID: 6485709 Document Number: 234077
The authors subjected 14 human sperm samples to decondensation in vitro. They analyzed the images of a total of 9,681 objects as a function of time of decondensation. The discussion bears on the heterogeneity of the process of chromatin decondensation in human spermatozoa, on the influence of the zinc/fructose ratio in the seminal fluid and on the role of the period during which the spermatozoa are left in contact with the fluid.