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Use of 5-bromodeoxyuridine for determining the duration of cell cycle phases

Chebotarev, A.N.; Selezneva, T.G.

Tsitologiia i Genetika 13(3): 163-167

1979

ISSN/ISBN: 0564-3783
PMID: 90414
Document Number: 149154
The method of differential staining of sister chromatids by means of 5-bromdesoxyuridine (BDU) was used to determine duration of the cellular cycle and its phases in a cell culture of Chinese hamster. The mitotic cycle lasted for 16 hrs, G1 for 5 hrs, S for 8 hrs and G2 for 3 hrs. These values do not differ from those obtained by the autoradiography. The given method permits quicker determination of the cellular cycle phases duration as compared with the autoradiography method.

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