Liquid-chromatographic measurement of urinary monosaccharides
D'Amboise, M.; Hanai, T.; Noël, D.
Clinical Chemistry 26(9): 1348-1350
1980
ISSN/ISBN: 0009-9147 PMID: 6772342 Document Number: 159607
We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D-fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.