Simplified liquid-chromatographic determination of vancomycin
Rosenthal, A.F.; Sarfati, I.; A'Zary, E.
Clinical Chemistry 32(6): 1016-1019
1986
ISSN/ISBN: 0009-9147 PMID: 3708801 Document Number: 272600
We simplified determination of vancomycin in serum by using a direct and rapid solvent precipitation of protein and by using a simple organic compound as internal standard. Reproductibility of the vancomycin retention time was improved by modifying the mobile phase. Data obtained by fluorescence polarization immunoassay correlated well, and there was little bias between the two methods. The present method is sufficiently rapid to make this a practical choice for many laboratories.