Liquid chromatographic determination of tolnaftate in commercial products
Thompson, R.D.; Carlson, M.
Journal - Association of Official Analytical Chemists 74(4): 603-607
1991
ISSN/ISBN: 0004-5756 PMID: 1917806 Document Number: 370533
A liquid chromatographic (LC) method for the determination of the antifungal agent tolinaftate was developed. Isolation of the analyte was achieved by direct extraction or dilution with acetonitrile-water (80 + 20) followed by reverse-phase liquid chromatography using a C18 column. The mobile phase was acetonitrile-water (80 + 20) acidified with phosphoric acid. Detection was by UV absorption at a wavelength of 257 nm. The proposed procedure was applied to 20 consumer products comprising 6 formulation types, including solutions, powders, liquid and powder aerosols, creams, and gels. The precision (RSD) for the products ranged from 0.23 to 1.16% (n = 5), and recoveries via fortification ranged from 98.1 to 103.0%. Six different brands of C18 columns were evaluated for use with the method. The overall simplicity and versatility of the method suggest possible adaptations to both regulatory and quality-control situations.