Liquid-chromatographic determination of nadolol in plasma
Gupta, R.N.; Haynes, R.B.; Logan, A.G.; Macdonald, L.A.; Pickersgill, R.; Achber, C.
Clinical Chemistry 29(6): 1085-1087
1983
ISSN/ISBN: 0009-9147 PMID: 6851099 Document Number: 220041
We describe a liquid-chromatographic procedure for determining nadolol in plasma. After an analog of nadolol is added as internal standard, the plasma sample is passed through a disposable BondElut C18 column. After several column washes, nadolol and the internal standard are eluted with methanol, and the eluate is evaporated and reconstituted with the mobile phase (acetonitrile/water, perchloric acid, and tetramethylammonium hydroxide). An aliquot of the extract is chromatographed on a non-silica resin-base reversed-phase column. The peaks are detected by fluorescence (lambda ex = 265 nm and lambda em = 305). Drug and internal standard are well resolved, and only a few extraneous peaks appear. The standard curve ranges from 10 to 400 micrograms/L. We are using this procedure to determine steady-state concentrations of nadolol in patients receiving various dosages of nadolol along with other types of antihypertensive drugs.