Top Abstract Purchase Related
Home
>
Node
>
Subnode

Globin synthesis in erythroid cultures from normal and thalassemic subjects

Perrotta, C.; Bernardi, F.; Conconi, F.; Delazer, A.; Del Senno, L.; Marchetti, G.

Bollettino della Societa Italiana di Biologia Sperimentale 56(11): 1133-1136

1980

ISSN/ISBN: 0037-8771
PMID: 7448020
Document Number: 159310
Relative alpha and beta chain synthesis in erythroid bursts derived from human bone marrow and peripheral blood are presented. The biosynthetic ratios obtained are compared with those of the reticulocytes. The data obtained cell culture are similar to the "in vivo" ones, showing and alpha/beta ratio of 1 in normal individuals and higher than 1 in beta thalassemic heterozygous. The use of erythroid cell culture as an experimental model for the study of differentiation at molecular level is therefore possible.

Document emailed within 1 workday
Secure & encrypted payments

Kirschmann, C.; Zaizov, R.; Steinhertz, M.; Matoth, Y. 1975: Globin chain synthesis and distribution in erythroid precursors and in reticulocytes of alpha-thalassemic patients Israel Journal of Medical Sciences 11(11): 1222
Gambari, R. 1983: Hexamethylenebisacetamide-mediated induction of murine erythroleukemia cells: relationship between expression of beta major and beta minor globin genes, globin mRNA synthesis and commitment to erythroid differentiation Biochemistry International 7(4): 471-480
Izzo, P.; Corciulo, R.; Mossa, V.; Costantino, R. 1977: Microchromatography for determination of hemoglobin a 2: statistical study of values in normal subjects and beta-thalassemic heterozygotic subjects Bollettino della Societa Italiana di Biologia Sperimentale 53(10): 787-791
Efremov, G.D.; Huisman, T.H. 1979: Preferential synthesis of fetal hemoglobin in in vitro cultures of erythroid precursors from peripheral blood of healthy persons and those with hemoglobinopathies Bilten Za Hematologiju i Transfuziju 7(1 Suppl): 39-45
Mulligan, R.C.; Howard, B.H.; Berg, P. 1979: Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome Nature 277(5692): 108-114
Cirotto, C.; D'Amelio, V. 1974: Globin pattern of adult chicken erythroid cells. Chromatographic analysis Bollettino della Societa Italiana di Biologia Sperimentale 50(12): 865-870
Peresleni, T.Iu.; Mechetner, E.B.; Rozinova, E.N.; Dvorkin, G.A. 1982: Transcription of globin gene in erythroid cells transformed by Rauscher virus Biokhimiia 47(2): 312-316
Enver, T.; Zhang, J.W.; Brice, M.; Karlinsey, J.; Papayannopoulou, T. 1989: Synkaryons: surrogate erythroid cells for the analysis of human globin gene switching Progress in Clinical and Biological Research 316b: 69-81
Kimura, T.; Mills, F.C.; Allan, J.; Gould, H. 1983: Selective unfolding of erythroid chromatin in the region of the active beta-globin gene Nature 306(5944): 709-712
Perrine, S.P.; Miller, B.A.; Papayannopoulou, T.; Faller, D.V.; Rudolph, A.M.; Vichinsky, E.; Hurst, D.; Kan, Y.W. 1989: Butyrate analogues modulate globin gene expression in human and ovine erythroid cells Progress in Clinical and Biological Research 316b: 341-350
Ginder, G.D.; Burns, L.J.; Glauber, J.G.; Beck, J. 1989: Pharmacologic induction of embryonic globin gene expression in adult avian erythroid cells Progress in Clinical and Biological Research 316b: 327-339
Ottolenghi, S.; Mantovani, R.; Nicolis, S.; Ronchi, A.; Malgaretti, N.; Giglioni, B.; Gilman, J. 1989: Altered binding to the gamma-globin promoter of two erythroid specific nuclear proteins in different HPFH syndromes Progress in Clinical and Biological Research 316a: 229-236
Huot, R.I.; Nardone, R.M.; Stromberg, K. 1987: Differential modulation of human chorionic gonadotropin production by methotrexate in normal and malignant placental cultures and its increase by dibutyryl cyclic adenosine monophosphate and/or actinomycin D in normal cultures Cancer Research 47(2): 383-387
Carlson, D.P.; Ross, J.; Wierenga, P.K.; Nijhof, W. 1987: Expression of the 5'-flanking region of the beta major-globin gene in cultured murine erythroid precursor cells Experimental Hematology 15(8): 902-907
Roth, E.F.; Shear, H.L.; Costantini, F.; Tanowitz, H.B.; Nagel, R.L. 1988: Malaria in beta-thalassemic mice and the effects of the transgenic human beta-globin gene and splenectomy Journal of Laboratory and Clinical Medicine 111(1): 35-41
Goncharenko, I.D.; Pavlov, A.D. 1982: Synthesis of nuclear and mitochondrial DNA and RNA and heme and globin synthesis in the bone marrow of rabbits with stimulated erythropoiesis Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia 3: 36-39
Stoeckert, C.J.; Green, M.B. 1994: Erythropoietin and hydroxyurea can act on early erythroid progenitors from adult human peripheral blood to modulate fetal globin mRNA levels Experimental Hematology 22(3): 278-282
Gillery, P.; Serpier, H.; Polette, M.; Bellon, G.; Clavel, C.; Wegrowski, Y.; Birembaut, P.; Kalis, B.; Cariou, R.; Maquart, F.X. 1992: Gamma-interferon inhibits extracellular matrix synthesis and remodeling in collagen lattice cultures of normal and scleroderma skin fibroblasts European Journal of Cell Biology 57(2): 244-253
Bank, A.; Acuto, S.; Giambona, N.; Donovan-Peluso, M. 1987: Regulation of human globin gene expression in human erythroid cells Progress in Clinical and Biological Research 251: 221-233
Guasco, G.; Gamalero, P.C.; Cuniolo, A. 1985: Psychological problems of thalassemic subjects La Pediatria Medica e Chirurgica: Medical and Surgical Pediatrics 7(2): 271-278