Transcription of globin gene in erythroid cells transformed by Rauscher virus
Peresleni, T.Iu.; Mechetner, E.B.; Rozinova, E.N.; Dvorkin, G.A.
Biokhimiia 47(2): 312-316
1982
ISSN/ISBN: 0320-9725 PMID: 6175349 Document Number: 187747
The synthesis of globin mRNA in cultured murine erythroleukemic cells transformed by the Rauscher leukemia virus and in the spleen of mice with Rauscher erythroleukemia was studied. The content of globin RNA sequence was estimated by cDNA hybridization with increasing amounts of cytoplasmic and poly (A)-containing RNA Globin RNA was found in the spleen of mice with Rauscher erythroleukemia, its content in cytoplasmic RNA and poly (A)-containing RNA being 0.02% and 0.4%, respectively. By contrast, no globin RNA sequences were found in transformed cultured murine erythroleukemic cells, which can be a cause of altered hemoglobin synthesis in these cells.