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Double-helical structures in the make up of poly (A) -containing RNA of rat liver cells. Effect of cortisone

Konstantinova, I.M.; Kulichkova, V.A.; Petukhova, O.A.; Vorob'ev, V.I.

Molekuliarnaia Biologiia 14(6): 1354-1361

1980

ISSN/ISBN: 0026-8984
PMID: 7442674
Document Number: 155126
Cytoplasmic poly(A)-containing RNA from rat liver cells are shown to contain RNase-resistant double-stranded regions. Their content increases after cortisone injection. The isolated double-stranded (ds) regions in polyacrylamide gel electrophoresis are seen as discrete bands of three size classes. Their melting curves are typical for double-stranded nucleic acids. The ds-structure isolated from poly(A)+-RNA of cortisone-stimulated rat livers has higher thermostability, with that of control livers. After denaturation 90% of ds-regions become single-stranded. About 50% of denatured double-stranded fragments reassociate at the range of COT = 10(-3)-10(-2). The origin of ds-structures in cytoplasmic poly(A)+-RNA and their possible functions are discussed.

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