Immunological assay of double-helical segments in RNA fractions of different molecular size extracted from acute myeloid leukemia blast cells
Torelli, U.; Ferrari, S.; Montagnani, G.; Torelli, G.; Cadossi, R.; Ferrari, S.; Narni, F.
Cancer Research 37(8 Part 1): 2667-2672
1977
ISSN/ISBN: 0008-5472 PMID: 68812 Document Number: 117084
Whole-cell RNA, extracted from acute myeloid leukemia blast cells, was fractionated by sedimentation through sucrose gradients. The proportion of double-helical segments present in each fraction was then determined by a quantitative microcomplement fixation assay that specifically measures double-helical RNA. Sizable amounts of double-helical segments were detected in all fractions of cellular RNA corresponding to S values higher than approximately 20. In all cell populations examined the highest proportion of double-helical segments was found in RNA fractions sedimenting faster then the 45 S ribosomal precursors RNA, i.e., in fractions including only heterogeneous nuclear RNA.