The specific features of the immunophenotype of blast cells in patients with de novo normal karyotype acute myeloid leukemia and FLT3-ITD mutation
Gritsaev, S.V.; Martynkevich, I.S.; Chubukina, Z.V.; Petrova, E.V.; Kostroma, I.I.; Ivanova, M.P.; Martynenko, L.S.; Potikhonova, N.A.; Bubnova, L.N.; Abdulkadyrov, K.M.
Terapevticheskii Arkhiv 86(3): 71-77
2014
ISSN/ISBN: 0040-3660 PMID: 24779074 Document Number: 676327
To study the specific features of de novo acute myeloid leukemia (AML) with FLT3-ITD mutation. The results of examination were analyzed in 101 patients. Bone marrow morphological specimens were stained with Pappenheim stain. The karyotype was investigated using the standard GTG-banding method. Blast cells were immunotyped in a five-color analysis on a Cytomics FC 500 laser flow cytofluorometer. FLT3-ITD mutation was identified in 21 patients who had a varying morphological nature of blasts, different karyotype variants, and frequently additional NPM1 gene mutation. The distinctive property of 10 patients with normal karyotype and FLT3-ITD mutation (without NPM1 gene mutation) was the larger number of cases with high expression of HLA-DR and CD7 than in the control group that included 18 patients with normal karyotype AML without FLT3-ITD nutation: 50% versus 6.2% (p = 0.007) and 100% versus 55.6% (p = 0.014), respectively. Normal karyotype AML with FLT3-ITD mutation is a group that is homogeneous in the biological phenotype of leukemia cells.