Lysosomes of blast cells in various cytochemical types of acute leukemia
Mdzewski, B.; Lawkowicz, W.; Kolakowska-Polubiec, K.; Litwin, J.; Grabarczyk, M.; Rokicka, R.; Bielecka, B.
Acta Haematologica Polonica 8(1): 6-13
1977
ISSN/ISBN: 0001-5814 PMID: 848251 Document Number: 113114
In 25 cases cytochemical types of acute leukemia were determined using the classification of Loeffler. Three cytochemical methods used were: p.a.S.-glycogen content, pox-peroxidase activity, and esterase activity determinatione. At the same time selective identification of lysosomes in perypheral blood blast cells was done using vital euchrisine staining and fluorescence microscopy. A correlation was observed between the cytochemical types of acute leukaemia and the lysosomal pattern. The fluorescence method of the identification of lysosomes is suggested for diagnosis of acute leukaemia.