Effects of histone fractions on induction of differentiation of cultured mouse myeloid leukemia cells
Okabe-Kado, J.; Honma, Y.; Hayashi, M.; Hozumi, M.
Cancer Research 41(5): 1997-2002
1981
ISSN/ISBN: 0008-5472 PMID: 7214367 Document Number: 169963
Mouse myeloid leukemic cells (M1) could be induced to differentiate into macrophage-like and granulocyte-like cells by a lysine-rich, histone H1 fraction (10 to 100 microgram/ml). The differentiated M1 cells expressed phagocytic and lysozyme activity and were macrophage-like and granulocyte-like cells. The differentiation-inducing activity of histone H1 was found in histone H1 fractions isolated from calf thymus, rat liver, and mouse leukemia M1 cells. Histone H2A and H2B fractions did not induce differentiation of M1 cells at concentrations of 10 to 100 microgram/ml but did induce differentiation at a high concentration (200 microgram/ml). The histone H3 fraction, poly-L-lysine and poly-L-arginine, inhibited induction of differentiation of M1 cells.