A rapid neutralization test for antibodies to bovine leukemia virus, with the use of rhabdovirus pseudotypes
Závada, J.; Cerný, L.; Závadová, Z.; Bozonová, J.; Altstein, A.D.
Journal of the National Cancer Institute 62(1): 95-101
1979
ISSN/ISBN: 0027-8874 PMID: 214611 Document Number: 140298
Two rhabdoviruses, vesicular stomatitis (type Indiana) and Chandipura viruses, formed pseudotype particles with envelope antigens provided by bovine leukemia virus (BLV). The pseudotypes were infectious for calf, human, mink and rat cells, but the most sensitive indicator was Vero [African green monkey kidney] cells. Infectivity of the pseudotypes was increased by DEAE-dextran present during adsorption. Sera of spontaneously infected cattle contained high titers (some over 1/10,000) of antibodies neutralizing the pseudotypes, whereas sera of cattle from uninfected herds possessed no neutralizing activity in 1/10 dilution. The neutralization of these pseudotypes can serve as a rapid and sensitive test for the detection of antibodies in cattle infected with BLV.