Cross-reactions of normal bovine serums to foot-and-mouth disease virus in plaque-reduction neutralization and radial immunodiffusion
Andersen, A.A.
American Journal of Veterinary Research 36(7): 979-983
1975
ISSN/ISBN: 0002-9645 PMID: 167625 Document Number: 85871
Serum samples from 150 cattle with no known exposure to FMD virus were tested by both the plaque-reduction neutralization (PRN) technique and the radial immunodiffusion (RID) technique to evaluate the significance and the extent of cross-reactions in these tests. Serum samples from 30 cattle from each of five locations were tested against representative viruses of each of the seven types of FMD virus. High levels of cross-reactions with both the RID and PRN techniques were found in samples from specific groups. Higher levels of cross-reactions were noted for Asia, SAT I, SAT II, and SAT III than for A, O, or C viruses with the PRN technique, and higher levels for SAT II and SAT III with the RID technique. The reactions for most samples appeared to be specific; a given serum was often reactive with only one or two viruses.