Nature of the dietary carbohydrate and metabolism of glycosaminoglycans and glycoproteins in rats
Menon, P.V.; Kurup, P.A.
Journal of Nutrition 106(4): 555-562
1976
ISSN/ISBN: 0022-3166 PMID: 176334 Document Number: 102878
The polysaccharide from mung bean or black gram (Phaseolus mungo) affected cholesterol, phospholipid and triglycerides in rats on high-cholesterol diets with high (NAR 44, 2305) or low (NAR 45, 2223) contents of fat. Male albino rats weighing 60 g were fed for 3 months on diets of casein 16, carbohydrate 68, groundnut oil 8, cellulose 3, vitamins 1, salts 4; the carbohydrate consisted of glucose alone (control), sucrose 60 + purified wheat starch 8, or black gram polysaccharide. The pattern of change of different glycosaminoglycans varied in different tissues. Rats given sucrose had less sulphated glycosaminoglycans in aorta and liver. Rats given polysaccharide and glucose gave comparable values in aorta except that chondroitin sulphate B was higher and heparin lower in the polysaccharide group. L-Glutamine:D-fructose-6-phosphate amino transferase and UDPG dehydrogenase were lowest in rats given sucrose and highest in the polysaccharide group, the glucose group showing intermediate values, but UDPG pyrophosphorylase, while highest in the polysaccharide group, was similar in the glucose and sucrose groups. Some of the degrading enzymes studied, beta -glucuronidase, hyaluronidase and aryl sulphatase, were highest in the sucrose group and generally lowest in the polysaccharide group. Values of 3'-phosphoadenosine-5'-phosphosulphate, the biological sulphating agent, the sulphate activating system that included ATP sulphurylase and APS kinase and sulphotransferase activity were lowest in the sucrose group and highest in the polysaccharide group. Glycoprotein concentration was highest in liver and lowest in kidney in the sucrose group.