A nuclease specific for depurinated DNA in germinating pea seeds
Wani, A.A.; Hadi, S.M.
Indian Journal of Biochemistry and Biophysics 13(3): 307-309
1976
ISSN/ISBN: 0301-1208 PMID: 1010581 Document Number: 99117
An enzymatic activity specific for depurinated DNA was identified in germinating pea seeds. Crude extracts prepared from germinating seeds produced acid-soluble diphenylamine positive material in the presence of depurinated DNA while the formation of such material from normal, and single-stranded, DNA was comparatively low. The maximum acid-soluble material produced from depurinated DNA was not increased by a subsequent treatment with alkali, indicating that the enzymatic action is mostly directed towards apurinic sites. The apurinic site nuclease activity could be observed in dry and imbibed seeds.