Proteins of polyribosome-bound informosome of germinating wheat embryos
Iskakov, B.K.; Aĭtkhozhin, M.A.
Molekuliarnaia Biologiia 13(5): 1124-1129
1979
ISSN/ISBN: 0026-8984 PMID: 503061 Document Number: 147916
Proteins of polyribosome-bound informosomes of germinating wheat embryos were studied by polyacrylamide gel electrophoresis with sodium dodecyl sulfate. Liberation of informosomal proteins was achieved by mild RNase treatment of polyribosomes. Proteins of informosomes associated with polyribosomes contain polypeptides with MW of 86,000, 75,000, 72,000, 66,000, 52,000 and 34,000. The MW of 2 most prominent proteins were 86,000 and 52,000. The treatment of polyribosomes with 0.5 M KCl resulted in the loss of a large part of informosomal proteins, which are revealed in the KCl-wash.