Use of proteins, covalently bound to agar gel, for specific sorption in electrophoresis
Kuzovleva, O.B.; Nikolaeva, A.I.
Voprosy Meditsinskoi Khimii 22(3): 413-417
1976
ISSN/ISBN: 0042-8809 PMID: 67710 Document Number: 100396
A method is developed for electrophoretic fractionation of serum proteins in agar gel with simultaneous specific sorption of gamma -globulin fraction, migrating during the electrophoresis through specific immunosorbents incorporated into agar gel. The reaction was in a special apparatus designed for the electrophoresis in 2 perpendicular directions. The sorbent was separated from impurities by passing currents transversely. The unspecific sorbent did not alter the electrophoretic mobility of migrating proteins, but the specific sorbents immobilized completely both antibodies and antigens. The antibodies bound repeatedly to immunoglobulins which had been linked to the sorbent. The method distinctly facilitated the estimation of antigens and antibodies in complex mixtures. By analogy with affinity chromatography the term "affinity electrophoresis" was used for describing the method.