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Effect of nuclease S1 on DNA of the highly oncogenic simian adenovirus SA7 (C8)

Zavizion, B.A.; Naroditskiĭ, B.S.; Grodnitskaia, N.A.; Makhov, A.M.; Tikhonenko, T.I.

Molekuliarnaia Biologiia 16(3): 569-574

1982

ISSN/ISBN: 0026-8984
PMID: 6285177
Document Number: 183457
The effect at specific nuclease S1 on DNA and the complex viral DNA-terminal protein of the highly oncogenic simian adenovirus SA7(C8) was studied. It was shown that nuclease S1 did not digest the bound between DNA and terminal protein in the complex but residual amino acid(s) was cleaved out after digestion with pronase. The DNA obtained after nuclease S1 action could be ligated and its 5'-ends were phosphorylated by polynucleotide kinase.

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