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Detailed study of the structure of simian adenovirus genome DNA and identification of its sequence in transformed cells

Denisova, T.S.; Gibadulin, R.A.

Voprosy Virusologii 27(5): 77-83

1982

ISSN/ISBN: 0507-4088
PMID: 7147908
Document Number: 190497
The paper describes physical maps of SA-7P DNA drawn with the help of XhoI, HpaI and BglII enzymes. DNA nucleotide sequence in the left side of the viral genome was found to have the length of 150 nucleotides. It contains codon for initiation of protein synthesis and coincides in this area with the known primary structure of Ela operon for human adenoviruses Ad5, Ad7, and Ad12. Study on the cells of two lines transformed by fragments of SA-7P DNA showed DNA of these cells to contain elements of the viral genome in different numbers of copies.

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