Characteristics of integration of monkey adenovirus SA7 DNA fragments with genomes of transformed and tumoral cells
Chaplygina, N.M.; Gartel', A.L.; Ponomareva, T.I.; Tiunnikov, G.I.; Tikhonenko, T.I.
Voprosy Meditsinskoi Khimii 29(3): 14-22
1983
ISSN/ISBN: 0042-8809 PMID: 6308902 Document Number: 212614
Transformed rat cells and hamster tumor cells obtained after treatment with DNA from monkey adenovirus SA7 which was fragmented using endonuclease Sa1L, contained all the fragments of SA7 DNA integrated with in the cell genomes. As shown by blotting hybridization distinct deletion did not form in the fragments of viral DNA on integration. Insertion of the SA7 DNA fragments appears to occur into a definite relatively small site of the cell chromosome. In the both cell strains studied a site of the SA7 genome transcribed at coordinates 1.8-10 units of the physical map. Fragments of virual DNA were methylated at the sites CmCGG and CCmCGGG of both strains studied.