RNA biosynthesis in the microsomes of splenic cells during the inductive phase of the immunological response
Perelygin, N.L.; Perelygina, O.V.; Babichev, V.A.; Uteshev, B.S.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 29-33
1975
ISSN/ISBN: 0372-9311 PMID: 1210907 Document Number: 93547
RNA labeled with 3H-uridine was extracted from the microsomes of the spleen of the intact and antigen-stimulated mice. A study was made of the composition of this RNA by electrophoresis in polyacrylamide gel. Apart from rRNA, there were revealed in the microsome composition up to 10 RNA components in the mol wt range of from 0.4-10(5) to 7-10(5) dalton and 2 componnents - between 7-10(5) and 1.7-10(6) dalton. Incorporation of 3H-uridine into the rRNA was the maximal 24 hours after the administration of the antigen, whereas the RNA with the mol wt between 0.4-10(5) dalton remaining practically unchanged for a period of three days after the immunization. 3H-uridine incorporation into these RNA was resistant to the action of low antibiotic (actinomycin D) doses. Immunization was not accompanied by the appearance of new, by molecular weight, RNA components.