Permeation-increasing activity of puncture-fluids: 2. Chromatographic and immunoelectrophoretic studies
Liebe, S.; Wagner, J.; Rogos, R.
Zeitschrift für die Gesamte Innere Medizin und ihre Grenzgebiete 30(23): 763-768
1975
ISSN/ISBN: 0044-2542 PMID: 1083598 Document Number: 92941
A human pleural effusion was separated by gel chromatography, DEAE-chromatography, disk-electrophoresis and immunoelectrophoresis. The fractions obtained were tested for the permeation-increasing activity. IgA, IgM, IgG, transferrin and haptoglobin are not responsible for a permeation-increasing effect. Albumin, alpha1-antitrypsin, acid alpha1-glycoprotein and alpha2-macroglobulin appear only in permeation-active fractions. Of these proteins, however, no direct effect on the permeability of animal membranes is known. It is discussed, whether proteolytic enzymes, which may be bound to these proteins are responsible for the increase of permeation.