Isoelectric focusing of pure rat pancreatic juice in polyacrylamide gel : possibility of concomitant estimation of radioactivity incorporated into single identified protein constituents
Desharnais, M.; Paradis, D.; Morisset, J.; Deaudoin, A.; Dunnigan, J.
Revue Canadienne de Biologie 34(1-2): 59-64
1975
ISSN/ISBN: 0035-0915 PMID: 1178942 Document Number: 89632
A method is described for the isoelectric focusing of rat pancreatic juice on columns of polyacrylamide gel containing urea. Twenty-six bands were separated, and 6 were identified. Application of this method of separation to in vivo radioactively labelled pancreatic juice resulted in bands which could be counted with precision. This procedure allowed also the expression of radioactivity of one constituent relative to any single band, or relative to total radioactivity, concomitantly.