Fractionation of "tricholysine" by isoelectric focusing
Stepanova, T.N.; Iulikova, E.P.; Maksimova, R.A.; Silaeva, A.B.; Andreenko, G.V.
Biokhimiia 42(1): 167-170
1977
ISSN/ISBN: 0320-9725 PMID: 557999 Document Number: 111998
A possibility to fractionate fibrinolytically and thrombolytically active complex "tricholysin" into five components, differing in iso-points and in enzymatic activity, is demonstrated by means of isoelectric focusing using ampholine solution within pH range 3.0-10.0. Homogenous fraction IV (isopoint 6.8-7.0) has a low caseinolytic activity and a high specific fibrinolytic and esterase activities. This fraction is characterized with a high ability of plasminogen activation. Serine, threonine, alanine and valine are found to prevail in amino acid composition of the fraction IV, its molecular weight being 39000.