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Cysteine adducts of human haemoglobin measured by isoelectric focusing in polyacrylamide gels with a non-linear pH gradient

Evelo, C.T.; Niessen, H.J.; Roelofs, H.M.; Henderson, P.T.

Journal of Chromatography 420(1): 35-42

1987

ISSN/ISBN: 0021-9673
PMID: 3667828
Document Number: 289062
The in vitro formation of adducts from human haemoglobin formed by alkylation with methylmethanesulphonate, dimethyl sulphate and iodoacetamide was determined with isoelectric focusing in ultra-thin polyacrylamide gels with a non-linear pH gradient. The most important adduct seen in the gels was identified as HbA alkylated at the .beta.-93 cysteine. Influences of the chemical nature of the alkylating agents and of the biological environment are discussed. The method is suggested to be applicable to monitoring the biological effects of low, long-term exposure to mixtures of alkylating agents or of exposure to unknown compounds.

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Porter, D.R.; Weeks, J.T.; Anderson, M.P.; Guenzi, A.C. 1992: An easy technique for extruding polyacrylamide gels from isoelectric focusing tubes of 1.0- to 1.5-mm inside diameter Biotechniques 12(3): 380-382
Alonso, A.; Gascó, P. 1987: The use of separator isoelectric focusing in micro-ultrathin polyacrylamide gels in the characterization of some polymorphic proteins of forensic science significance Journal of Forensic Sciences 32(6): 1558-1564
Gustafsson, J.A.; Gustafsson, S.A.; Nordenskjöld, B.; Okret, S.; Silfverswärd, C.; Wrange, O. 1978: Estradiol receptor analysis in human breast cancer tissue by isoelectric focusing in polyacrylamide gel Cancer Research 38(11 Part 2): 4225-4228
Kazanets, E.G.; Zakharova, F.A.; Levina, A.A.; Andreeva, A.P.; Tokarev, I.N. 1982: Polyacrylamide gel isoelectric focusing for the study of erythrocyte methemoglobin reductase Problemy Gematologii i Perelivaniia Krovi 27(11): 56-58
Tumakov, S.A.; Pervushkin, S.V. 1977: Replication-applications in the isoelectric focusing in the thin polyacrylamide layer Laboratornoe Delo 7: 408-410
Abe, O. 1981: A simple analytical isoelectric focusing apparatus with sucrose density gradient Seikagaku. Journal of Japanese Biochemical Society 53(12): 1299-1301
Syresina-Nedugova, G.I.; Rubtsov, I.V.; Martynov, I.V. 1979: Fractionation of Vibrio cholerae enterotoxin by isoelectric focusing in an ampholin gradient Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 110-111
Good, A.H.; Ceverha, B.B. 1971: Immunologic assays for identifying single components in protein mixtures after isoelectric focusing in urea-containing acrylamide gels Journal of Immunology 106(6): 1677-1680
Leskovar, P.; Kratzer, M. 1979: Electrophoretic determination of individual urinary protein fractions. new technic of microelectrophoresis on continuous polyacrylamide gradient gels Die Medizinische Welt 30(47): 1777-1782
Desharnais, M.; Paradis, D.; Morisset, J.; Deaudoin, A.; Dunnigan, J. 1975: Isoelectric focusing of pure rat pancreatic juice in polyacrylamide gel : possibility of concomitant estimation of radioactivity incorporated into single identified protein constituents Revue Canadienne de Biologie 34(1-2): 59-64
Trbojević-Cepe, M.; Poljaković, Z.; Franjić, J.; Bielen, I.; Vranes, Z. 1989: Detection of oligoclonal IgG bands in unconcentrated CSF in multiple sclerosis and other neurological diseases by isoelectric focusing on ultrathin-layer polyacrylamide gel immunofixation and silver staining Neurologija 38(1): 11-21
Bozhko, G.K.; Voloshin, P.V.; Kostiukovskaia, L.S.; Kulabukhov, V.M. 1983: Use of disc electrophoresis in a linear gradient of polyacrylamide gel concentrations for simultaneous fractionation of total serum proteins, haptoglobins and lipoproteins Ukrainskii Biokhimicheskii Zhurnal 55(3): 318-324
Alsayed, N.; Drüeke, T.B.; Lacour, B. 1988: Purification of anti-apolipoprotein CIIi antibodies by exploiting their affinity for apolipoprotein CIIi linked to polyacrylamide gel after isoelectric focusing Clinical Chemistry 34(1): 49-52
Sutton, J.G. 1979: Further alleles of phosphoglucomutase in human semen detected by isoelectric focusing Journal of Forensic Sciences 24(1): 189-192
Wetzel, V.; Schaumlöffel, E. 1976: Isoelectric focusing of an alkylating 3H-cyclophosphamide derivative from human bladder urine International Journal of Clinical Pharmacology and Biopharmacy 14(4): 279-283
Rasulov, E.M.; Movsum-zade, K.M. 1985: Separation and identification of human globin chains by the method of isoelectric focusing Laboratornoe Delo 9: 527-530
Farmer, P.B.; Bailey, E.; Green, J.A.; Leung, C.S.; Manson, M.M. 1991: Biomonitoring of human exposure to alkylating agents by measurement of adducts to haemoglobin or DNA Iarc Scientific Publications 105: 71-77
Pellikka, P.A.; Sullivan, W.P.; Coulam, C.B.; Toft, D.O. 1983: Comparison of estrogen receptors in human premenopausal and postmenopausal uteri using isoelectric focusing Obstetrics and Gynecology 62(4): 430-434
Thomas, J.W.; Virta, V.J.; Nell, L.J. 1985: Heterogeneity and specificity of human anti-insulin antibodies determined by isoelectric focusing Journal of Immunology 134(2): 1048-1052
Leung, F.Y.; Henderson, A.R. 1981: Isolation and purification of aspartate aminotransferase isoenzymes from human liver by chromatography and isoelectric focusing Clinical Chemistry 27(2): 232-238