Standardization of Dermatophagoides pteronyssinus extracts and tyrosine adsorbates
Dewdney, J.M.; Hordle, D.A.; Munro, A.C.; Shah, C.
Developments in Biological Standardization 29: 378-388
1975
ISSN/ISBN: 0301-5149 PMID: 238873 Document Number: 86706
The human skin test, inhibition of radioallergosorbent test (RAST) and the radioimmunosorbent technique of Ceska have been shown to be useful assays for the standardisation of aqueous extracts of Dermatophagoides pteronyssinus (Trt.) The development of these assay systems for the standardisation of D. pteronyssinus adsorbed on to tyrosine is described. Tyrosine solubilisation procedures are detailed together with the influence of these procedures on the three assays. Good correlation has been shown between the human skin test and the radioimmunosorbent technique. The inhibition of RAST appears to be more sensitive than the other techniques to mild conformational changes in the allergen, induced in this work by an acid pH. This may limit its value for the standardisation of D. pteronyssinus adsorbed on to tyrosine, but it may prove useful in monitoring manufacturing processes.