A complex study of strains of human cells with karyotype anomalies. III. An immunoelectrophoretic analysis of water-soluble antigens
Mikhaĭlov, A.T.; Kuliev, A.M.
Genetika 11(10): 117-124
1975
ISSN/ISBN: 0016-6758 PMID: 1225745 Document Number: 85943
Antisera to diploid, trisomic and triploid embryonic fibroblast-like cells were obtained after hyperimmunization of rabbits. Immunoelectrophoretic analysis with these antisera revealed up to 9 water-soluble antigens in embryonic cells, which were present in skin fibroblasts from adult donors as well. Trisomic and triploid strains did not differ from the diploid ones by the spectrum of water-soluble antigens. The content of the number of antigens (especially of cathode fractions) in trisomic cells was significantly low as compared with those in control diploid cells, whereas in triploid ones it differed slightly. All the strains were characterized by the presence of proteins immunologically identical to alpha-globulin of human serum.