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Determination of the number of photoreactivating enzyme molecules per haploid Saccharomyces cells

Yasui, A.; Laskowski, W.

International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 28(6): 511-518

1975

ISSN/ISBN: 0020-7616
PMID: 1082862
Document Number: 85397
Two haploid radiation-sensitive mutants of Saccharomyces were studied to investigate the formation of a complex between photoreactivating-enzyme and substrate after UV irradiation. Using photo-flashes, the time necessary for maximum complex formation was determined. Within 1 min, 70% of the complexes were formed. To determine the number of photoreactivating enzyme molecules per cell, the maximum dose decrement obtained after 1 photo-flash was determined and corrected for the effects of non-photoreactivable lesions. The corrected maximum dose decrement was identical for both strains (8.5 erg mm-2). The number of photoreactivating-enzyme molecules involved in the photorepair of nuclear DNA damage was calculated as 272 .+-. 27.

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