Determination of the concentration of ligand-specific molecules, found in mixtures with ligand-nonspecific molecules
Bobrovnik, S.A.
Ukrains'kyi Biokhimichnyi Zhurnal 72(1): 113-119
2000
ISSN/ISBN: 0372-3909 PMID: 10979570 Document Number: 512548
A new method, which allows determination of the concentration of ligand-specific molecules in a mixture of these molecules with biochemically similar but ligand-unspecific molecules, is suggested. The method is based on a partial exhaustion of the mixture on a column with immobilized ligand and determination of the part of ligand-specific molecules presented in exhausted mixture. The concentration of monoclonal antibodies specific to bovine serum albumin in a commercial "Sigma" preparation and concentration of polyreactive immunoglobulins in a commercial "Sigma" preparation of bovine immunoglobulins were determined by suggested method.