A natural specific intracellular ligand as a reagent for cyclosporine blood level determination
Hasková, V.; Rozprimová, L.; Svobodová, J.
Folia Biologica 37(2): 134-139
1991
ISSN/ISBN: 0015-5500 PMID: 1874341 Document Number: 373964
We isolated and characterized a non-immunoglobulin fraction from calf thymuses which was found very active in binding [3H]labelled cyclosporine (CsA). It contains the natural, intracellular receptor cyclophilin, specifically binding to the active site of the CsA molecule, which may also play a part in the immunosuppression of human immunocompetent cells. This material with a high selectivity could be used, instead of specific monoclonal or polyclonal antibodies, to determine CsA levels in patient's blood.