Actin from diploid and haploid cells of the myxomycete Physarum flavicomum--comparison with skeletal muscle actin
Henney, H.R.; Hubbell, C.
Microbios 23(91): 25-34
1978
ISSN/ISBN: 0026-2633 PMID: 42005 Document Number: 131911
Glycerinated diploid microplasmodia of Physarum flavicomum exhibited ATP-dependent contractility typical of actomyosin controlled systems. Actin was isolated from microplasmodia, haploid amoebae-swarm cells, and rabbit skeletal muscle, by classical methods as well as ATP-DEAE cellulose chromatography, and isoelectric focusing. Actins from all three sources had isoelectric points in the pH 6.5-7.0 range, and behaved similarly during the various isolation procedures. SDS-gel electrophoresis revealed that the actin from microplasmodia and muscle had a molecule weight of about 46,000 daltons while the amoebae-swarm cells possessed proteins of molecular weights of about 46,000 and 51,000 daltons.