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Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene

Zang, Y.-m.; Li, Y.-p.; Qiao, J.; Chen, H.-h.; Liu, C.-s.

Yao Xue Xue Bao 50(7): 906-909

2015

ISSN/ISBN: 0513-4870
PMID: 26552155
Document Number: 684386
β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.

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