Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode
Zhang, H.-Y.; Chen, J.; Jia, J.; Liu, D.; Shi, L.-C.; Zhang, H.; Song, J.-Y.; Yao, H.
Zhongguo Zhong Yao Za Zhi 39(12): 2208-2211
2014
ISSN/ISBN: 1001-5302 PMID: 25244746 Document Number: 674740
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.