Role of PI3K/Akt pathway in effect of paeoniflorin against Aβ25-35-induced PC12 cell injury
Liu, L.; Wang, S.-Y.; Wang, J.-G.
Zhongguo Zhong Yao Za Zhi 39(20): 4045-4049
2014
ISSN/ISBN: 1001-5302 PMID: 25751960 Document Number: 674741
To study the role of PI3K/Akt pathway in the neuroprotective effect of paeoniflorin on PC12 cells. The paeoniflorin group (5, 10, 20 μmol · L(-1)) was pretreated for 30 min, and then added with Aβ25-35 (20 μmol · L(-1)) for interaction for 24 h. Inhibitor LY294002 (10 μmol · L(-1)) was pretreated for 30 min before the action of paeoniflorin (10 μmol · L(-1)). The MTT colorimetric method was used to detect the cell viability. The apoptosis rate was tested by the FITC-Annexin V/PI staining. The protein expression of p-AKT, Bax, Bcl-2 and cleaved caspase-3 protein were detected by Western blot analysis. Paeoniflorin could significantly inhibit the Aβ25-35-induced PC12 cell toxicity and apoptosis. Its protection effect may be achieved by up- regulating AKT phosphorylation level, increasing Bcl-2 protein expression, reducing Bax protein expression, inhibiting the activation of caspase-3. Inhibitor LY294002 could weaken the above protective effects of paeoniflorin. Paeoniflorin could activate PI3K/Akt signaling pathway to protect the PC12 cell injury induced by Aβ25-35.