Protective effect of qingxin kaiqiao fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35
Ye, G.-H.; Hu, H.-Y.; Chen, X.; Wang, W.-H.; Zhang, X.-Y.
Zhongguo Zhong Yao Za Zhi 37(21): 3271-3274
2012
ISSN/ISBN: 1001-5302 PMID: 23397728 Document Number: 656672
To observe the protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35, in order to provide basis for clinical application of the formula. Sprague Dawley rats were orally administration with Qingxin Kaiqiao Fang (7.9 g x kg(-1)) twice a day for 3.5 days to prepare Qingxin Kaiqiao Fang containing cerebrospinal fluid. The nerve cell injury model was established by PC12 cells and Abeta25-35 with the concentration of 10 micromol x L(-1). The expressions of Bax, Bcl-2 and Caspase-3 mRNA were detected by immunohistochemical method in the PC12 cells. The Qingxin Kaiqiao Fang group showed a significant higher PC12 cell activity than the model group, with decrease in Bax mRNA and Caspase-3 mRNA expressions and increase in Bcl-2 mRNA expression. There was a significant difference from the model group (P < 0.05, P < 0.01). Qinxin Kaiqiao Fang shows a significant protective effect on Abeta25-35-induced nerve cell injury.