Studies on identification of drugs of abuse by diode array detection. I. Screening-test and identification of benzodiazepines by HPLC-DAD with ICOS software system

Shimamine, M.; Masunari, T.; Nakahara, Y.

Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 111: 47-56

1993


ISSN/ISBN: 0077-4715
PMID: 7920567
Document Number: 419808
For the establishment of screening-test and identification of 20 benzodiazepines (Alprazolam, Bromazepam, Chlordiazepoxide, Clonazepam, Clotiazepam, Cloxazolam, Diazepam, Estazolam, Fludiazepam, Flunitrazepam, Flurazepam, Lorazepam, Lormetazepam, Medazolam, Midazepam, Nimetazepam, Nitrazepam, Oxazepam, Prazepam, Triazolam), the optimum separation condition on HPLC was investigated by using Interactive Computer Optimization for HPLC Separation (ICOS) software. The two eluent systems of 0.02M KH2PO4 (pH 3.1)-methanol-acetonitrile (66.4:5.7:27.9) and 0.1% TFA-methanol-acetonitrile (61.6:16.1:22.3) were selected by HPLC analyses using ICOS. These optimum separation conditions enabled the screening test and identification of the 20 benzodiazepines on HPLC with photodiode array detection.

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